Biochem/physiol Actions

Cell permeable: no

Reversible: no

Primary TargetCam Kinase-2

Target IC₅₀: 50 nM for both the total and Ca2+-independent CaMK II activity

Product does not compete with ATP.

General description

A potent and specific inhibitor of CaMK II (IC₅₀ = 50 nM for both the total and Ca²⁺-independent CaMK II activity). Exhibits inhibitory activity across converged species, including rat brain, goldfish brain, and Drosophila (IC₅₀ range 100-400 nM). Does not affect the activities of CaMK I, CaMK IV, CaMKK, PKA, or PKC. The peptide sequence corresponds to the inhibitory domain of CaMK IIN.

A potent and specific inhibitor of calmodulin kinase II (CaM-KII) (IC₅₀ = 50 nM for both total and Ca²⁺-independent activities of CaM-KII). Does not affect the activities of CaM-KI, CaM-KIV, CaM-KK, PKA, or PKC. The peptide sequence corresponds to the inhibitory domain of the inhibitory protein, CaM-KIIN. A cell-permeable version is available under Cat. No. 208921.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Soderling, T.R., et al. 2001. J. Biol. Chem.276, 3719.Chang, B.H., et al. 1998. Proc. Natl. Acad. Sci. USA95, 10890.Pereda, A.E., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13272.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.

Sequence

H-Lys-Arg-Pro-Pro-Lys-Leu-Gly-Gln-Ile-Gly-Arg-Ala-Lys-Arg-Val-Val-Ile-Glu-Asp-Asp-Arg-Ile-Asp-Asp-Val-Leu-Lys-OH

Warning

Toxicity: Standard Handling (A)